Enzyme Mixtures with Bromelain, Papain, Trypsin:

Precise enzyme activity assays

Medicines or natural remedies that are to activate the immune system and support the healing process of inflammation, often contain enzyme mixtures of bromelain, papain, trypsin or lysozyme. Due to the overlapping activities of bromelain, papain and trypsin, it was previously not possible to detect the individual activities of these enzymes in the mixtures. The BAEE- or Casein-Assay is not suitable for determining the activities of bromelain and papain in mixtures. We have established a new method in our laboratory that solves this problem.

We use artificial substrates and specific inhibitors, with which a differentiation of bromelain, papain and trypsin is possible. The catalytic activity of lysozyme can also be measured in mixtures with the established assay.

Comparative measurements with international standards allow the conversion of the activities into the international customary unit F.I.P. We analyze three samples of each batch in three tests (n = 9). This allows for a result specified with standard deviation.

Powders are suspended in a physiological solution and diluted until a proportionality between sample quantity and enzyme activity is achieved. To measure particle-bound enzyme fractions, the suspensions are not centrifuged.